LOCUS       40924_36468        4325 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway recycling vector pRED419 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Kimura T, Nakao A, Murata S, Kobayashi Y, Tanaka Y, Shibahara K, 
            Kawazu T, Nakagawa T.
  TITLE     Development of the gateway recycling cloning system for multiple 
            linking of expression cassettes in a defined order, and direction on
            gateway compatible binary vectors
  JOURNAL   Biosci. Biotechnol. Biochem. 77 (2), 430-434 (2013)
  PUBMED    23391940
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Kimura T, Shibahara K, Tanaka Y, Nakao A, Kawazu T, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 4325)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4325)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2013"; volume: "77"; issue: "2"; 
            pages: "430-434"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pDONR201.
FEATURES             Location/Qualifiers
     source          1..4325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     159..175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(188..244)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(245..261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    803..927
                     /label=attR4
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        952..982
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    1254..1302
                     /label=rare cutter sites
                     /note="rare cutter sites"
     protein_bind    complement(1569..1692)
                     /label=attR3
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2198..2297)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2420..3226
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3372..3960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4095..4122)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4214..4300)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga taagcaatgc ttttttataa tgccaacttt gtacaaaaaa
      121 gcaggctgga tgaatcgaat aacttcttct tcaaatgcca ggaaacagct atgaccatga
      181 ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
      241 attcactggc cgtcgtttta caacgtcgtg actgggaaaa cgcaaacgaa gaaatttcct
      301 cagtctcagc cacagccgcc ggagaaaaat gagaaaaaac tgaaaaggga gagatttccg
      361 gtgaaccctt tgagagattt cagtacaaga agctctggaa gcagcagttg tagcaatgag
      421 agtgcttctg gttcaacttc cggtgaagca tccaacggtt gtcacaggtt cttgctctct
      481 cattctttct catcgtcttc ttcatcatct tcttcttcgt tgggagtttt tcctcgtaga
      541 ccagtgagat cagtagctaa aaaccccaaa tctgctccgg tagttagtaa acccttaatt
      601 agaaagaaac caagctctct tgaggaggtt aagttgaaat ctactcttac tgagaaacct
      661 aatttgctga agtctcagag atgcaagaca aatcctgtat ctggaaagag accaacttgt
      721 aagattacca tgaaaccgga gaaagtttcg gttttgaaga aacaatcttc tgtttctagc
      781 tagaactagt ggatccccca tcacaacttt gtatagaaaa gttgaacgag aaacgtaaaa
      841 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      901 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      961 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccggcgag attttcagga
     1021 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1081 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1141 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1201 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attgcgatcg
     1261 catttaaatg gccggccctt aattaaggcg cgccgtttaa acgggcgacg gatggtgatc
     1321 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     1381 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     1441 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     1501 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
     1561 cgatcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     1621 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcaactttat
     1681 tatacaaagt tgtgatatca agctagcttc gacaattatt ttggactctc atgttctcaa
     1741 gctgaaatcc aaaagaaaca tgatgttggt tcagatttgg aatcactgac aatgaagttt
     1801 cagtcagccg gtttgtcacc aaggatccag gcttcatcat gggagccgag ccgttcaagt
     1861 ttcaacttcg actatttggc cacatcttct gattctattg atctctcaca gtttcagaga
     1921 ggtttagttg acaggagttc ttgtcatcct catgtaacac tggataaagt gtcaaggaca
     1981 catttgagag tagagcaaac aaatagccac atgcctgaaa tgaaatccca acagattaca
     2041 gatactgaat ttgatattca aaatcacaaa gaaagtgctg cttctctggg aaaaggaaga
     2101 gaattgcttc catgctctgc tgcagagtca ataagcactg atggaggtgg tctgatttgt
     2161 tctgaggatt caaattggat ggcatgttac aagaattacc cagctttctt gtacaaagtt
     2221 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     2281 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg
     2341 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2401 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2461 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2521 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     2581 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2641 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     2701 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     2761 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     2821 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     2881 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     2941 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3001 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3061 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3121 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3181 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3241 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3301 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3481 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3601 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3661 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3721 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3781 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3841 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3901 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3961 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4021 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag ccaggaagag
     4081 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4141 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4201 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4261 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4321 ctcgc
//
